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Cells in the cornea may be used to measure intraocular pressure (IOP). A device that measures IOP using cells in the cornea is commercially available (e.g., from Medtronic PhacoFx®).
These cells may also be used to measure blood flow velocity to evaluate the state of the eye. For example, care of the eye can be monitored by repeatedly measuring the number of cells in the cornea that migrate, between two consecutive measurements, as a function of time. This migration, which occurs due to blood flow, may be used as a measure of the blood flow velocity in the cornea. In an exemplary method, a baseline number of cells are counted over at least a minute. Then, the number of cells in the cornea is measured over the next minute. The rate of migration of the cells may be calculated, and a change in this migration rate compared to the baseline migration rate may be used to measure a change in blood flow velocity in the cornea.
Although the cells in the cornea can be used to measure blood flow, their ability to measure blood flow is limited by the ability of the eye to open. In particular, the cells may count more during a recording period when the eye is open. The eye may open when the cell migration rate is measured; however, a change in the migration rate is only observed when the cell migration rate is in the middle of the recording period.Expression of the cyclin G1 gene in Saccharomyces cerevisiae: the significance of the NH2-terminal sequence.
Numerous studies indicate that the NH2-terminal sequence of cyclin G1
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